percoll solution Search Results


acrylamide aam  (Chem Impex International)
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Chem Impex International acrylamide aam
Acrylamide Aam, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percoll solution  (Pharmacia Upjohn LLC)
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Pharmacia Upjohn LLC percoll solution
Percoll Solution, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percoll solution  (Biochrom)
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Biochrom percoll solution
Percoll Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percoll cell isolation solution  (Beijing Solarbio Science)
90
Beijing Solarbio Science percoll cell isolation solution
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percoll ® reagent  (Biochrom)
90
Biochrom percoll ® reagent
Percoll ® Reagent, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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low-density percoll solution  (Pharmacia Upjohn LLC)
90
Pharmacia Upjohn LLC low-density percoll solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Low Density Percoll Solution, supplied by Pharmacia Upjohn LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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low-density percoll solution - by Bioz Stars, 2026-06
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percoll solution  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc percoll solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Percoll Solution, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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70% percoll solution  (Merck KGaA)
90
Merck KGaA 70% percoll solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
70% Percoll Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percoll solution  (Beijing Solarbio Science)
90
Beijing Solarbio Science percoll solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Percoll Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/pmc08968607-84-26-17?v=Beijing+Solarbio+Science
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percoll separator liquid  (Promega)
90
Promega percoll separator liquid
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Percoll Separator Liquid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/pmc03272684-46-28-31?v=Promega
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percoll solution density  (Beijing Solarbio Science)
90
Beijing Solarbio Science percoll solution density
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Percoll Solution Density, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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percoll separating solution  (Biochrom)
90
Biochrom percoll separating solution
CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using <t>Percoll</t> solution 1·075 g/dl, layered <t>over</t> <t>1·035</t> g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).
Percoll Separating Solution, supplied by Biochrom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using Percoll solution 1·075 g/dl, layered over 1·035 g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).

Journal:

Article Title: Studies of delayed systemic effects of ultraviolet B radiation (UVR) on the induction of contact hypersensitivity, 2. Evidence that interleukin-10 from UVR-treated epidermis is the critical mediator

doi: 10.1046/j.1365-2567.2000.00934.x

Figure Lengend Snippet: CH‐inducing capacity of dendritic cells obtained from lymph nodes from IL‐10‐treated mice. Panels of mice received IL‐10 (200 ng) injections intradermally into abdominal skin. Eight hours later, lymph nodes not draining the injected skin (all except axillary and inguinal) were removed. Lymphoid cell suspensions were separated on density gradients using Percoll solution 1·075 g/dl, layered over 1·035 g/dl solution and centrifuged. Interface cells were collected and placed in Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded. The adherent cells were then incubated at 37° overnight and non‐adherent cells were collected as cell suspension enriched for DCs. As positive control, cells were prepared from lymph nodes of untreated mice. DC‐enriched suspensions derivatized with DNFB in vitro were injected into footpads of naive syngeneic mice (2 × 104 cells in 100 µl per mouse). Five days later CH was assayed as described in the legend to Fig. 1. Bars represent mean ear‐swelling responses ± SEM for groups of five mice each. Asterisks indicate mean responses significantly less than the positive control (P < 0·0005).

Article Snippet: The cells were then separated on density fractions using low‐density Percoll solution (Pharmacia & Upjohn, Peapack, WN) (1·035 g/dl), layered over a high‐density solution (1·075 g/dl), and centrifuged at 570 g for 20 min. Interface cells were collected and placed in 10‐ml Petri dishes at 37° for 60 min, after which non‐adherent cells were discarded.

Techniques: Injection, Incubation, Positive Control, In Vitro